Services

The facility provides fee for service analysis for a wide variety of applications.   This can be done on a “full service” basis that includes sample preparation, data collection and analysis or we can provide training and access to instrumentation and computers/software so that individual investigators can process their own samples and analyze their own data.

We can conduct accurate, high resolution mass analysis of small molecules and proteins.

We can perform quantitative measurements of small molecules using HPLC MS/MS on triple quadrupole or quadrupole time of flight instruments or, in cases where HPLC MS/MS is not suitable we can make these measurements using GC MS

We have assays for many commonly studied lipids, metabolites and pharmaceuticals.  Assays for analytes that we have not already worked with can be established on an individual basis.

Examples of molecules or classes of molecules that we can currently measure include:

Analyte Class

Analyte

Notes

Sterols, cholesterol esters

Sterols (Cholesterol, lanosterol, Squalene)

 

Cholesterol esters (~30 abundant species)

 

Oxysterols (10 species)

Dimethylglycine Derivatives

Neutral Lipids

Monoacylglycerols

 

Diacylglycerols (15 species/run)

 

Triacylglycerols(15 species/run)

 

Glycero phospholipids

Phosphatidylcholines/Lysophosphatidylcholines(15 species/run)

 

Phosphatidylserines(15 species/run)

 

Phosphatidylethanolamines(15 species/run)

 

Phosphatidylinositols(15 species/run)

 

Phosphatidic acids/ Lysophosphatidic Acids(15 species/run)

 

Phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-tris phosphate

Phosphomethyl derivatives

Sphingo phospholipids

Sphingomyelins(15 species/run)

 

Ceramides/Dihydroceramides(15 species/run)

 

Sphingosine/Dihydrosphingosine

 

Sphingosine 1-phosphate/Dihydrosphingosine 1-phosphate

 

Free fatty acids and derivatives

Fatty acids(15 species/run, can also analyze by infusion)

Methylpyridinium derivatives

Fatty acyl coAs(15 species/run)

 

Fatty Acyl Carnitines(15 species/run)

 

Prostaglandins & oxidized fatty acids

Prostaglandin F1 alpha, Tetranor prostaglandin E metabolite

Thromboxane B2, 15-keto Prostaglandin E2, Prostaglandin D2, Prostaglandin E2, 6-Keto Prostaglandin F1 alpha, F and J type Isoprostanes, resolvin D1, resolvin D2, maresin, DiHDoHE, HETE, Lipoxin A4

 

Polyphenols & nutritional protectants

ECGC, Resveratrol

 

Environmental Toxins

PCB 4, PCB 77, PCB 118, PCB 126, PCB 153, 2-hydroxy pentachlorobiphenyl (2-OH PCB), biphenyl, and 2-phenyl phenol.